phosphokinase antibody array  (R&D Systems)

Journal: Foods

Article Title: Structural Elucidation of Heteropolysaccharides from the Peach-Shaped Dictyophora indusiata and Its Anti-Inflammatory Activity

doi: 10.3390/foods14091536

Figure Lengend Snippet: Effects of DIVP on the human cytokines and phosphokinase. ( A , B ) Dot blot array analysis ( A ) and quantification ( B ) of pro-inflammatory cytokine secretion from LPS-stimulated U937-macrophages in the presence or absence of 0.2 mg/mL DIVP. ( C , D ) Dot blot array analysis ( C ) and relative quantification ( D ) of human phosphokinase from LPS-stimulated U937 macrophages in the presence or absence of 0.2 mg/mL DIVP. A, B, C, D, E in Fig. 6A represent different rows. Statistical significance was determined with a one-way or two-way ANOVA (for dot blot assay), and p values are as follows: ns, not significant; * p < 0.05, ** p < 0.01 vs. control group; # p < 0.05, ## p < 0.01 vs. LPS group.

Article Snippet: Protein phosphorylation was evaluated using phosphokinase antibody array assays (ARY003C, R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions.

Techniques: Dot Blot, Quantitative Proteomics, Control

Journal: Theranostics

Article Title: FAM3C in circulating tumor-derived extracellular vesicles promotes non-small cell lung cancer growth in secondary sites

doi: 10.7150/thno.72297

Figure Lengend Snippet: Transcriptomic and molecular perturbations in FAM3C overexpressed cells. RNA-Seq analysis of FAM3C_ox cells versus control cells. (A) Volcano plot of log 2 (fold change) and -log 10 (adjusted p-value) of all identified genes between control and FAM3C-overexpressing cells. (B) The top 10 enriched disease ontology (left panel) and KEGG pathway (right panel). (C) GSEA analysis of significantly enriched genes in FAM3C-overexpressing cells vs the control cells. NES, normalized enrichment score; FDR, false discovery rate. The red color scale indicates the positive and blue color indicates the negative correlation. (D) Histograms plotting the gene targets within the significantly enriched GSEA pathways. (E) Key targets genes from the RNA-Seq were validated using quantitative PCR (qPCR) analysis. (F-H) Kinome profiling of FAM3C_ox cells. (F) Human phospho-kinase array analysis was performed in H2170 and FAM3C_ox cells. Red box, blue box, green box and orange box indicates the phosphoproteins of Src, ERK1/2, Akt (T308) and STAT3 (Y705) respectively. (G) Bar graph represents the fold change expression of each phosphokinase proteins relative to control cells. (H) Western blot analysis of the indicated targets in H2170 and FAM3C_ox cells. Beta actin was used as a loading control.

Article Snippet: Human phosphokinase antibody array kits were obtained from R&D Systems® to analyze the phosphorylation profiles of kinases according to manufacturer's instructions ( https://www.rndsystems.com/resources/technical/assays-analytes-represented-human-phospho-kinase-array-kit ).

Techniques: RNA Sequencing, Control, Real-time Polymerase Chain Reaction, Expressing, Western Blot